Crystallization and preliminary X-ray diffraction studies of the pneumococcal teichoic acid phosphorylcholine esterase Pce.

Crystallization and preliminary X-ray diffraction studies of choline-binding protein F from Streptococcus pneumoniae.

Choline-binding protein F (CbpF) is a modular protein that is bound to the pneumococcal cell wall through noncovalent interactions with choline moieties of the bacterial teichoic and lipoteichoic acids. Despite being one of the more abundant proteins on the surface, along with the murein hydrolases LytA, LytB, LytC and Pce, its function is still unknown. CbpF has been crystallized using the han...

Insights into molecular plasticity of choline binding proteins (pneumococcal surface proteins) by SAXS.

Phosphocholine moieties decorating the pneumococcal surface are used as a docking station for a family of modular proteins, the so-called choline binding proteins or CBPs. Choline recognition is essential for CBPs function and may also be a determinant for their quaternary structure. There is little knowledge about modular arrangement or oligomeric structures in this family. Therefore, we have ...

Modular Architecture and Unique Teichoic Acid Recognition Features of Choline-Binding Protein L (CbpL) Contributing to Pneumococcal Pathogenesis

The human pathogen Streptococcus pneumoniae is decorated with a special class of surface-proteins known as choline-binding proteins (CBPs) attached to phosphorylcholine (PCho) moieties from cell-wall teichoic acids. By a combination of X-ray crystallography, NMR, molecular dynamics techniques and in vivo virulence and phagocytosis studies, we provide structural information of choline-binding pr...

Pneumococcal serotypes and their clinical relevance.

Pneumococcal disease is endemic all over the world. Epidemics may occur, most often in closed institutional settings such as schools and military camps, but account for only a minority of all the cases. The total incidence of pneumococcal pneumonia in Western populations is 1–5 per 1000 person-years, and several times higher in the very young and the elderly. In developing countries it is estim...

Moieties of Pneumococcal Teichoic Acid Human L-Ficolin Recognizes Phosphocholine